BactoCount IBCm Series: Bacteria & Somatic Cells Counter

BactoCount IBCm Series: Bacteria & Somatic Cells Counter

Real-Time enumeration of individual Bacteria & Somatic Cells in raw milk

Semi-Automatic version up to 50 analysis/hour

The new BactoCount IBCm 3.0 is a semi-automated instrument that uses a proprietary process based on next-generation flow cytometry for the rapid, highly accurate, reliable and multiplex enumeration of individual bacteria and somatic cells in raw milk.
The rapid test for bacteria and somatic cells makes it the ideal solution for any processing plant or laboratory involved in the quality assessment of milk.

The instrument offers multiplex applications thanks to its unique dedicated platform. Up to two applications can be run simultaneously on a given sample. The overall design of the instrument has also been designed to allow ease of access and maintenance. With this in mind, IBCm 3.0 embeds a new kinetic, self aligned flow cell mounting system.

The BactoCount IBCm 3.0 can be equipped with a conveyor, automating the sampling at a maximum rate of 50 analysis/hour. The sampling, sequencing and identification of the sample vials are functions performed by the Autosampler.

Validated to be at least equivalent to the EN-ISO 4833-1:2013 and 4833-2:2013 reference methods for the enumeration of microorganisms in raw cow milk (Certificate n° 2013LR44)

Compatible with the ILAS 4000 ROBOT: Automation System for Bacteria Counter

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Features & Benefits

Features & Benefits

For milk quality control at Reception

ISO 16140-2:2016 certified
Real-time and highly accurate determination of raw milk hygienic quality in compliance with ISO 16140 standard
Unique high speed solution for multiplex automated testing
Highly standardised and reproducible method guaranteeing worldwide equivalence of results across laboratories and countries
Highly accurate and robust universal conversion equation based on a very large and representative samples database
Excellent instrument standardisation and quality control with our long shelf life lyophilised standards (IBC and SCC) and microspheres working solution.

Powerful to enhance milk value

Milk payment, screening and segregation
Enable dairies to comply with EU Commission Regulation
Real-time detection of milk tanker contamination before unloading
Real-time detection of milk silo contamination before processing
Segregation of the milk as a function of its quality
Improve end-products consistency
Fast return on investment.

Specifications

Specifications

	BactoCount IBCm*
Raw milk type	Cow, goat, sheep, buffalo, …
Carry-over	≤ 1% (typically ≤ 0.5%)
Total flora	IBCm – ISO 16140-2:2016 certified IBCm 3.0 – equivalent to BactoCount IBCm Range: 2,000 – 10,000,000 IBC/mL
Repeatability	Range (IBC/μL) 10 – 50 51 – 100 101 – 300 > 300	Specifications Sr ≤ 0,07 log Sr ≤ 0,06 log Sr ≤ 0,05 log Sr ≤ 0,03 log
Reproducibility	Range (IBC/μL) 10 – 50 51 – 100 101 – 300 > 300	Specifications S_R ≤ 0.14 log S_R ≤ 0.12 log S_R ≤ 0.10 log S_R ≤ 0.06 log
Accuracy	Sy, x ≤ 0.3 log (ISO 4833) Cow: Sy, x = 0.167 (AIA) Sheep: Sy, x = 0.245 (AIA) Buffalo: Sy, x = 0.201 (AIA)
Somatic cells	0 – 10,000,000 cells (SCC)/mL
Sample Temperature	4 – 42 °C
Accuracy	≤ 10% (ISO 13366-1)
Repeatability	Range (SCC/μL) 100 – 300 300 – 500 > 500	Specifications Cv ≤ 5% Cv ≤ 3% Cv ≤ 2%
Technical Specifications
Speed	Analysing time: < 1 minute Sample prep time: IBCM <10s \| IBCM 3.0 automatic Incubation time: 1 minute (SCC)/<10 minutes (IBC)
Undiluted water factor	10 – 100
Voltage	110/220 V ; 50/60 Hz
Connected to the local database and remotely accessible

*Specifications subject to change without any prior notice.

Technical Overview

Technical Overview

The BactoCount System consists of 5 modules:

Computer
Autosampler
Fluid Handling
Open Incubator/Sonic probes
Industrial Flow Cytometer

For total flora analysis:

An incubation reagent consisting of a clarification buffer, a proteolytic enzyme, and a fluorescent marker is added to the milk in order to lyse the somatic cells, solubilize the fat globules and proteins, permeabilise the bacteria and stain their DNA.
The fluorescent marker intercalates rapidly and selectively into all the bacteria double-stranded nucleic acid.
The mixture is sonicated during the incubation period to help the chemical breakdown of the interfering particles, disrupt the remaining bacteria colonies to improve the detection of individual bacteria and reduce the background fluorescence.
After the incubation period, a portion of the incubation mixture is transferred to the flow cytometer where the bacteria are aligned and exposed to an intense laser beam and fluoresce.
The fluorescent signal is collected by the optics, filtered, and detected with a photomultiplier.
The intensity and height of the fluorescent pulses are recorded and used as gating parameters.
The sorted pulses (IBC) are then converted into Colony-Forming Units (CFU) after the application of a conversion equation.

For somatic cell analysis:

An incubation reagent consisting of a clarification buffer, and a fluorescent marker is added to the milk in order to permeabilise the somatic cells and stain their DNA.
The fluorescent marker intercalates rapidly and selectively into all the somatic cells double-stranded nucleic acid.
After the incubation period, a portion of the incubation mixture is
transferred to the flow cytometer where the somatic cells are aligned and exposed to an intense laser beam and fluoresce.
The fluorescent signal is collected by the optics, filtered, and detected with a photomultiplier.
The intensity and height of the fluorescent pulses are recorded and used as gating parameters.

flow-cytometery