Mammalian Cell Analysis

Mammalian Cell Analysis

Phenotype MicroArrays for Mammalian Cells

Using Biolog’s Phenotype MicroArray™ Technology, any type of cell can be characterised for metabolically related phenotypes in over 1,000 assays in a simple, rapid, and cost-effective manner.

By measuring a cell’s metabolic activity, including responses to a genetic alteration or chemical stimuli, this integrated system of cellular assays, instrumentation, and bioinformatic software reveals unique and insightful information on metabolic pathway activities, and cellular sensitivity to nutrients, hormones, cytokines, anti-cancer agents, and ions. By measuring energy output of cells, the technology also provides new assays for understanding mitochondrial function and toxicity. Phenotype MicroArrays enable insight and discovery to expedite scientific publications.

This is accomplished using 96 well microplates, pre-loaded with hundreds of KEGG pathway-matched metabolic substrates, and metabolic effectors.

Phenotype MicroArrays are a proven method of cellular screening that is beneficial in a wide range of research applications:

Genotype/phenotype studies
Cell line characterisation and QC
Cellular metabolism, metabolic disorders, nutrition
Cell energetics growth and death
Hormone effects on cells
Metabolic reprogramming in cancer, Warburg effect, anti-cancer drug sensitivity
Mitochondrial toxicology in chemical/drug screens
Stem cells and differentiation
Cell line and bio process development

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Phenotype Microarray™ Technology Overview

Biolog Mammalian Technology Overview from Biolog on Vimeo.

The OmniLog^® PM-M System Specifications

The OmniLog^® PM-M system includes: instrument, computer, LCD flat panel monitor, software for data collection and analysis, 8 & 12 channel electronic pipettors, user guide, training and one year warranty.
Consumables and other accessories are purchased separately.

Size: 21 in x 32 in x 23 in (53 cm x 81 cm x 58 cm)
Power: 100 to 240 volts, 50 to 60 Hz
Operating Temp Range: 18˚ to 28˚C
Operating Humidity Range: 20% to 80% non-condensing
Incubation Temperature Range: 20˚ to 45˚C
Temp Consistency: ±2˚C in the tray chamber
Incubation Humidity Range: Ambient
Test Capacity: 50 microplates
Data Capture: CCD images of each microplate at regular intervals
Temperature Control: Input of set temperature by external computer
Temperature Indication: Output to external computer and LED numeric display

Assay Portfolio for Mammalian Cells

Biolog PM-M Plate

Carbon Energy & Nitrogen Substrates: 367 metabolic substrates
Substrates include carbohydrates/starches, alcohols, fatty acids, ketones, carboxylic acids, amino acids and dipeptides on 4×96 well microplates.

Hormones
45 different hormones each at six different concentrations on 3×96 well microplates.

Trace Elements
22 different ions/cofactors each at four different concentrations on 1×96 well microplate.

Anti-Cancer Chemosensitivity
92 anti-cancer agents each at four different concentrations on 4×96 well microplates.

Mitochondrial Toxicity
8 carbon-energy substrates (associated with diverse pathways, e.g. hexose, pentose, etc.) to distinguish different aspects of mitochondrial activity/dysregulation in the context of chemical/drug screens on 1×96 well microplate.

Kinetic Data Capture and Analysis

The OmniLog™ software contains a suite of algorithms that work in conjunction with the OmniLog™ and the Phenotype MicroArray™ panels to automate incubation of up to 50 microplates at a user-specified temperature with continuous collection of colorimetric assay data over time. Data analysis programs allow for display of kinetic data, management and analysis of data, and facilitates export in a variety of raw and processed forms.

File Management/Kinetic/Parametric Analysis

Assembles plate or PM-M panel data files into data lists
Displays kinetic plots of the data
Allows export of kinetic plots as bmp or jpeg files
Extracts data lists from the File Management/Kinetic

Analysis module

Calculates parameters from kinetic data
Allows comparison of two data lists
Highlights wells and generates a report on phenotypes that differ significantly in any selected kinetic analysis parameter
Allows identification of substrates in all PM-M panels
Links metabolic substrates in PM-M panels to the KEGG database
Exports the original OmniLog kinetic data
Exports data parameters for statistical and bioinformatic analysis