Mammalian Cell Mitochondria Analysis

Mammalian Cell Mitochondria Analysis

Analysing Mitochondria at Higher Resolution with MitoPlate™ Technology

Analysing Mitochondria at higher resolution with Biolog Technology

MitoPlates™ provide a powerful new research tool by allowing scientists to run preconfigured sets of 96 mitochondrial function assays in one experiment. Mitochondria can be interrogated and characterised in novel ways, looking at rates of substrate metabolism, sensitivity to drugs and other chemicals, and effects of mutations in mitochondria-related genes.

Assay Principle
Mitochondrial function is assayed by measuring the rates of electron flow into and through the electron transport chain from metabolic substrates that produce NAD(P)H or FADH2 such as L-malate, succinate, pyruvate, etc. Each substrate follows a different route, using different transporters to enter the mitochondria and different dehydrogenases to produce NAD(P)H or FADH2, The electrons travel from the beginning (complex 1 or 2) to the distal portion of the electron transport chain where a tetrazolium redox dye (MC) acts as a terminal electron acceptor that turns purple upon reduction. Additional MitoPlate assays probe the sensitivity of the mitochondria to a set of 22 diverse inhibitors.

Investigate how mitochondria change with:

Cell differentiation
Cancer & Aging
Neurological disorders
Metabolic disorders
Immune cell activation
Bacterial/viral infection
Inborn genetic defects

Download Brochure

Mitochondrial Function Assay Technology Overview

New Probes of Mitochondrial Function from Biolog on Vimeo.

Simple Assay Procedure:

STEP 1: Prepare and pipet assay mixture containing cell permeabilising buffer and redox dye into appropriate wells.
STEP 2: Start the assays by adding 2x cell suspension to all wells.
STEP 3: Load the MicroPlate into the OmniLog® for kinetic reading of the rate of purple colour formation.

Biolog MitoPlate S-1 and I-1 Protocol (10-17-2018) from Biolog on Vimeo.

Unique Features and Advantages

An essential tool in your milk quality-assurance program.
MitoPlates are preloaded with 96 tests ready for use
Plates designed to measure effects of substrates and inhibitors on mitochondrial function
Easy, robust protocols with any cell type – adherent or suspension cells, transformed cell lines or primary cells
Assays need only 20,000 to 40,000 cells per well
Novel tetrazolium dye chemistry provides a terminal electron acceptor in easy to read colorimetric assays
OmniLog instrument provides automated temperature controlled incubation and kinetic reading of multiple plates (50 plates at 15 min, or 16 at 5 min intervals)

OmniLog System and Analysis Software

The OmniLog Instrument and associated software allows for real-time recording and kinetic analysis of electron flow rates. The OmniLog can simultaneously incubate and read up to 50 MitoPlates and provides powerful analysis tools to get the most from your experimental data.