RhizoPlates for Microbial Selection in Sustainable Agriculture

RhizoPlates™

Microbial Selection for Sustainable Agriculture

The RhizoPlate™ series of microplates provides a targeted and easy-to-use solution for detecting, ranking, and monitoring nitrogen-fixing & phosphate-solubilising bacteria (NFB/PSB) within the rhizosphere.

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Features:
Resources:

Features:

Features:

Advancing Sustainable Agriculture: Simplifying Biostimulant product development to reduce use of synthetic fertilisers.
Comprehensive Analysis: Select for and evaluate NFB/PSB capability from bacterial isolates, microbial communities, and complex soil samples. Measure growth under a range of conditions at the same time to identify and evaluate the most robust strains or communities.
Streamlined Relative Quantification: Kinetic optical density (OD) measurements based on the resultant growth from N2 or inorganic phosphate utilisation are used to rapidly assess and compare the performance of NFB/PSB, which is ideal for strain screening.
Persistence Monitoring: Track NFB/PSB in soil over time to guide cost-effective biostimulant dosing in the field.
Scalable Throughput: Seamlessly integrates with the Odin platform for small-scale (Odin VIII) or higher-throughput (Odin L) screening operations, with powerful software for actionable insights.

Turnkey Solution:

RhizoPlate N includes a 96-well plate pre-loaded with 30 carbon substrates in triplicate, specifically selected to support the unique growth requirements of NFB and a nitrogen-free inoculating fluid.
RhizoPlate P provides a 96-well microplate uniformly coated with insoluble inorganic phosphate, and pre-loaded with 30 carbon substrates in triplicate, selected to support the unique growth requirements of PSB and a phosphate-free inoculating fluid.

Resources:

Resources:

View RhizoPlate™ Flyer here

View RhizoPlate™ Map here

Applications

Screening and Selecting Isolates:

Rapidly screen through large strain collections
Rank candidates
Compare nitrogen fixation or phosphate solubilising performance
Select engineered strains to move forward for additional analysis

Communities:

Analyse complex soil microbial communities
Develop biostimulants that combine complementary species or strains for broader substrate utilisation
Evaluate and compare the performance of multiple microbial communities on a single, integrated platform

Monitoring Persistence in Soil:

Monitor the presence of Nitrogen Fixing Bacteria and Phosphate Solubilising Bacteria in agricultural fields
Track PSB/NFB persistence over time
Ensure optimal biostimulant dosing
Maintain cost-effectiveness in application of new biostimulant products

Workflow

A soil sample, community, or isolate is plated in triplicate across the microplates’ three identical sectors using their corresponding phosphate- or nitrogen-free IF.

The plate is transferred to the Odin instrument for incubation and kinetic OD590 measurements, where cells must use insoluble phosphate or fix nitrogen to grow.

Average Well-Growth Development (AWDG) data, calculated by Odin software, is used to screen phosphate solubilisation or nitrogen fixation performance across many samples easily.

Combined list of substrates

2,3-Butanediol Acetic Acid Adonitol
Citric Acid
D,L-α-Glycerol Phosphate
D,L-Lactic Acid
D-Fructose
D-Galactose
D-Galacturonic Acid
D-Gluconic Acid
D-Glucuronic Acid

D-Mannitol
D-Mannose
D-Saccharic Acid
D-Trehalose
Formic Acid
Glucose-6-Phosphate
Glycerol
Glycogen
i-Erythritol
L-Alanine

L-Arabinose
L-Asparagine
L-Aspartic Acid
L-Fucose
L-Glutamic Acid
L-Histidine
L-Phenylalanine
L-Proline
L-Pyroglutamic Acid
L-Rhamnose

Maltose
myo-Inositol
Polysorbate 80
Propionic Acid
Succinic Acid
Sucrose
Urocanic Acid
α-D-Glucose
α-D-Lactose
α-Keto-Valeric Acid