For use with our PCR rapid detection systems, BIOTECON Diagnostics has developed fast and easy-to-use DNA extraction kits. The foodproof® ShortPrep, StarPrep, Sample Preparation and Magnetic Preparation Kits conveniently assist you in efficiently working with all types of sample matrices, including difficult complex samples.
Sample Preparation Kits
ShortPrep: rapid purification
The ShortPrep I Kit is designed for quick and easy DNA extraction (using heat-induced lysis) from food enrichments of Gram-negative bacteria, including Salmonella.
Principle
- Cells are lysed using the foodproof ShortPrep I Kit lysis buffer in an incubation step.
- After centrifugation, the DNA is in the supernatant and ready to use.
Features
- User-friendly kit with 96 individual ready-to-use reactions for maximum efficiency and easy DNA extraction
- Optimized and validated for Gram-negative bacteria
- Suitable for a wide range of food, animal feed and environmental samples
- Validated and certified by internationally recognized institutions such as AOAC, NordVal International and DAFF
The ShortPrep II Kit is designed for quick and easy DNA extraction (using heat-induced lysis and mechanical disruption) from food enrichments of Gram-positive bacteria, including Listeria and Alicyclobacillus.
Principle
- Cells are lysed using the foodproof ShortPrep II Kit lysis buffer in an incubation step.
- After centrifugation, the DNA is in the supernatant and ready to use.
Features
- User-friendly kit with 96 individual ready-to-use reactions for maximum efficiency and easy DNA extraction
- Optimized and validated for Gram-positive bacteria
- Suitable for a wide range of food, animal feed and environmental samples
- Validated and certified by internationally recognized institutions such as AOAC, NordVal International and DAFF
StarPrep: bulk purification
The StarPrep One Kit is designed for quick and easy extraction of bacterial DNA from food enrichments by heat-induced lysis. This kit is a cost-effective bulk version, while the foodproof ShortPrep kits are pre-assembled in 96 ready-to-use tubes.
Principle
- Cells are lysed during incubation in the foodproof StarPrep One Kit lysis buffer.
- After centrifugation, the DNA is in the supernatant and ready to use.
Features
- Fast and flexible DNA extraction from food
- Successfully tested with hundreds of food matrices, animal feed and environmental samples including PPS
- Validated and certified by internationally recognized institutions such as AOAC, MicroVal, NordVal and DAFF
The StarPrep One 8-Strip Kit is designed for a quick and easy high-throughput extraction of bacterial DNA from Gram negative bacteria in food enrichments by heat-induced lysis.
Principle
- Cells are lysed in 8-strips by heat incubation in the foodproof StarPrep One Kit 8-Strip lysis buffer.
- After centrifugation, the DNA is in the supernatant and ready to use.
Features
- Fast and flexible high-throughput DNA extraction from Gram negative bacteria in all kind of foods
The StarPrep Two Kit is designed for quick and easy extraction of bacterial DNA from food enrichments by heat-induced lysis. This kit is a cost-effective bulk version, while the foodproof ShortPrep kits are pre-assembled in 96 ready-to-use tubes.
Principle
- Cells are lysed by mechanical disruption and incubation in the foodproof StarPrep Two Kit lysis buffer.
- After centrifugation, the DNA is in the supernatant and ready to use.
Features
- Fast and flexible DNA extraction from food and beverages
- Successfully tested with hundreds of food and beverages, animal feed and environmental samples
- Validated and certified by internationally recognized institutions such as NordVal and DAFF
The StarPrep Two 8-Strip Kit is designed for a quick and easy high-throughput extraction of bacterial DNA from Gram positive bacteria in food enrichments by heat-induced lysis.
Principle
- Cells are lysed by mechanical disruption and incubation in the foodproof StarPrep Two Kit 8-Strip lysis buffer.
- After centrifugation, the DNA is in the supernatant and ready to use.
Features
- Fast and flexible high-throughput DNA extraction from Gram positive bacteria in all kind of foods.
Enables a quick and easy high-throughput DNA extraction of yeast and mold in dairy products using 8 well strips. Besides mechanical and heat-induced lysis the protocol includes a differentiation of live and dead cells.
Principle
- Cells are treated with Reagent D* to eliminate the DNA of dead cells
- Then, cells are lysed by mechanical disruption and incubation in the foodproof StarPrep Six 8-Strip lysis buffer.
- After centrifugation, the DNA is in the supernatant and ready to use.
* needs to be purchased separately (Product No.: A 500 02)
Features
- Fast and flexible high-throughput DNA extraction from yeast & mold
- Tested with all kind of dairy matrices
- A live/ dead differentiation step is included.
The StarPrep Three Kit is designed for quick and easy extraction of bacterial DNA from food enrichments by heat-induced lysis. This kit is a cost-effective bulk version, while the foodproof ShortPrep kits are pre-assembled in 96 ready-to-use tubes.
Principle
- Cells are lysed during incubation in the foodproof StarPrep Three Kit lysis buffer
- After centrifugation, the DNA is in the supernatant and ready to use
Features
- Fast and flexible DNA extraction from enrichment samples
- Successfully tested with food matrices, seafood and environmental samples including PPS
The StarPrep Four Kit is designed for quick extraction of yeast DNA from food and beverage enrichments.
Principle
- Cells are lysed by mechanical disruption and incubation in the foodproof StarPrep Four Kit lysis buffer.
- After centrifugation, the DNA is in the supernatant and ready to use.
Features
- Fast and flexible DNA extraction
- Suitable for DNA extraction from yeast cells in beverage and food enrichments
The StarPrep Five Kit is designed for the quickest and easiest extraction of animal DNA. The entire workflow is performed in a single tube, minimizing handling steps and cross-contamination. The extraction Kit has been tested on many different food and feed samples and it also applicable on more complicated matrices such as gelatine.
Principle
- The 3 included buffers are mixed just before start of extraction.
- Fresh sample is added to the prepared buffer mix.
- Suspension is lysed by mechanical cell disruption and heat incubation.
- After centrifugation, the DNA is in the supernatant and ready to use.
Features
- Fast extraction of animal DNA from food samples with minimized handling steps and contamination risk.
Sample Preparation: high quality column purification
The Sample Preparation Kit I is designed for demanding isolation and purification of bacterial DNA from enriched food samples, including special matrices with a high proportion of PCR inhibitors. The kit is suitable for Gram-negative bacteria, such as Enterobacteriaceae, Salmonella, E. sakazakii, E. coli, Shigella.
Principle
- Cells are lysed in the foodproof Sample Preparation Kit I lysis buffer.
- The DNA is applied to the included glass fiber filter and purified with the foodproof Sample Preparation Kit I wash buffer.
- Purified DNA is then eluted using the foodproof Sample Preparation Kit I elution buffer and is ready to use.
Features
- Isolation of highly purified DNA, virtually free of other cellular components and PCR inhibitors
- Suitable for matrices containing PCR inhibitors
The Sample Preparation Kit II is designed for demanding isolation and purification of bacterial DNA from enriched food samples, including special matrices with a high proportion of PCR inhibitors. The kit is suitable for the detection of Gram-positive bacteria, such as Listeria and Alicyclobacillus.
Principle
- Cells are lysed by incubation in foodproof Sample Preparation Kit II lysis buffer and lysozyme.
- Extracted DNA is digested in proteinase K to destroy endogenous nucleases and other proteins.
- The DNA is applied to the included glass fiber filter and purified with the foodproof Sample Preparation Kit II wash buffer.
- Purified DNA is then eluted using the foodproof Sample Preparation Kit II elution buffer and is ready to use.
Features
- Isolation of highly purified DNA, virtually free of other cellular components and PCR inhibitors
- Suitable for matrices containing PCR inhibitors
The Sample Preparation Kit III is designed for the demanding isolation and purification of DNA from raw materials and foodstuffs of plant or animal origin for GMO, allergen or animal ID analysis by real-time PCR.
Principle
- Cells are lysed by incubation in the foodproof Sample Preparation Kit III extraction buffer.
- Extracted DNA is digested in proteinase K to destroy endogenous nucleases and other proteins.
- The DNA is applied to the included glass fiber filter and purified with the foodproof Sample Preparation Kit III wash buffer.
- Purified DNA is then eluted using the foodproof Sample Preparation Kit III elution buffer and is ready to use.
Features
- Isolation of highly purified DNA, free of other cellular components and PCR inhibitors
- The DNA is suitable for all qualitative and quantitative applications on all major real-time PCR devices
- Designed for all plant and animal matrices, including those containing genetically modified organisms
The foodproof Sample Preparation Kit IV is optimized for the isolation and purification of viral DNA and RNA from various food and water samples.
Principle
- The foodproof Sample Preparation Kit IV lysis buffer extracts the DNA / RNA out of virus particles.
- Absolute ethanol removes the hydration shell from nucleic acids.
- DNA / RNA is than bound to the glass fibers of pre-packed filter tubes.
- With the wash buffer I and II DNA / RNA will be cleaned and purified.
- At the final step DNA / RNA is recovered using the elution buffer and stabilized by a ribonuclease inhibitor.
Features
- Isolation of purified viral DNA / RNA.
- The DNA / RNA is suitable for all qualitative and quantitative applications on many real-time PCR devices.
- Variable sample-extraction volumes up to 500 µl.
- Designed and validated for many food matrices, including some with inhibitors (e.g. soft fruits and mussels).
Magnetic Preparation: automated purification
The Magnetic Preparation Kit I is designed for automated DNA extraction from enriched food samples of Gram-negative bacteria (e.g. Salmonella, Enterobacteriaceae, E. sakazakii, E. coli, Shigella) using magnetic beads.
Principle
- Cells are lysed by incubation in the Magnetic Preparation Kit I lysis buffer.
- Extracted DNA is bound to the magnetic beads, and purified with the foodproof Magnetic Preparation Kit I wash buffer to remove proteins and other cellular contaminants.
- Purified DNA is then eluted from the magnetic beads and is ready to use.
Features
- Innovative magnetic bead technology for the isolation of highly purified DNA
- Optimized and validated for a wide range of food matrices, animal feed and environmental samples
- Validated and certified in combination with devices from the foodproof RoboPrep Series by internationally recognized institutions such as AOAC and MicroVal
The Magnetic Preparation Kit II is designed for automated DNA extraction from enriched food samples of Gram-positive bacteria (e.g. Listeria spp.).
Principle
- Cells are lysed by incubation in the Magnetic Preparation Kit II lysis buffer.
- Extracted DNA is bound to the magnetic beads, and washed to remove proteins and other cellular contaminants..
- Purified DNA is then eluted from the magnetic beads and is ready to use.
Features
- Innovative magnetic bead technology for the isolation of highly purified DNA
- Optimized and validated for a wide range of food matrices, animal feed and environmental samples
- Validated and certified in combination with devices from the foodproof RoboPrep Series
The Magnetic Preparation Kit III is designed for automated plant and animal DNA extraction from food samples using magnetic beads. DNA is then ideal for qualitative and quantitative testing of genetically modified organisms, allergens, and species identification.
Principle
- Cells are lysed by the Magnetic Preparation Kit III lysis buffer.
- Extracted DNA is bound to the magnetic beads, and washed to remove proteins and other cellular contaminants.
- Purified DNA is then eluted from the magnetic beads and is ready to use.
Features
- Innovative magnetic bead technology for the isolation of highly purified DNA
- Optimized and validated for the detection of GMOs, allergens and animal species
- Validated in combination with the KingFisher™ Flex extraction robot
The Magnetic Preparation Kit IV is designed for automated DNA extraction of Gram-negative bacteria from enriched food samples (e.g. Salmonella, Enterobacteriaceae and Cronobacter) using magnetic beads.
Principle
- Cells are lysed by incubation in the foodproof Magnetic Preparation Kit IV Lysis Buffer.
- Extracted DNA is bound to the magnetic beads, and purified with the foodproof Magnetic Preparation Kit IV Wash Buffers to remove proteins and other cellular contaminants.
- Purified DNA is eluted with the foodproof Magnetic Preparation Kit IV Elution Buffer from the magnetic beads and is ready to use.
Features
- Innovative magnetic bead technology for the isolation of highly purified DNA.
- Optimized and validated for a wide range of food matrices, especially powdered infant formula (PIF) with and without probiotics and environmental samples.
- Suitable for PIF-testing in combination with the foodproof Enterobacteriaceae plus Cronobacter Detection Kit and Reagent D.
- MicroVal validation in combination with the foodproof Enterobacteriaceae plus Cronobacter Detection Kit and Reagent D and a robot from the foodproof RoboPrep Series.
