BactoCount IBCm Series: Bacteria & Somatic Cells Counter

Specifications

*Specifications subject to change without any prior notice.

Technical Overview

The BactoCount System consists of 5 modules:

1. Computer
2. Autosampler
3. Fluid Handling
4. Open Incubator/Sonic probes
5. Industrial Flow Cytometer

For total flora analysis:

• An incubation reagent consisting of a clarification buffer, a proteolytic enzyme, and a fluorescent marker is added to the milk in order to lyse the somatic cells, solubilize the fat globules and proteins, permeabilise the bacteria and stain their DNA.
• The fluorescent marker intercalates rapidly and selectively into all the bacteria double-stranded nucleic acid.
• The mixture is sonicated during the incubation period to help the chemical breakdown of the interfering particles, disrupt the remaining bacteria colonies to improve the detection of individual bacteria and reduce the background fluorescence.
• After the incubation period, a portion of the incubation mixture is transferred to the flow cytometer where the bacteria are aligned and exposed to an intense laser beam and fluoresce.
• The fluorescent signal is collected by the optics, filtered, and detected with a photomultiplier.
• The intensity and height of the fluorescent pulses are recorded and used as gating parameters.
• The sorted pulses (IBC) are then converted into Colony-Forming Units (CFU) after the application of a conversion equation.

For somatic cell analysis:

• An incubation reagent consisting of a clarification buffer, and a fluorescent marker is added to the milk in order to permeabilise the somatic cells and stain their DNA.
• The fluorescent marker intercalates rapidly and selectively into all the somatic cells double-stranded nucleic acid.
• After the incubation period, a portion of the incubation mixture is
  transferred to the flow cytometer where the somatic cells are aligned and exposed to an intense laser beam and fluoresce.
• The fluorescent signal is collected by the optics, filtered, and detected with a photomultiplier.
• The intensity and height of the fluorescent pulses are recorded and used as gating parameters.

Downloads

Download BactoCount IBCm 3.0 Product Sheet

Download Bentley Instruments General Catalog