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Protein Enzyme-Linked Immunosorbent Assay (ELISA) Tests for allergen proteins. The Neogen Allergen Protein ELISA Kits utilise a sandwich ELISA. The target protein present in the sample react with the specific antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antibodies conjugated with horseradish peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with the previously bound target protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The colour development from this enzymatic reaction varies directly with the concentration of target protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of the target protein in the test sample. The quantity of protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution. -
Lateral flow devices for specific allergen detection Streamline your process and simplify your allergen testing with 3Neogen Allergen Protein Rapid Tests – qualitative immunochromatographic assays for rapid in-plant monitoring of specific food allergens. Designed for accurate detection of processed and unprocessed allergen proteins, these fast, easy tests can be used for clean-in-place (CIP) final rinse water, environmental swab samples, raw ingredients and finished food products. We’ve taken the hassle out of allergen testing. You see the results. -
Detects as little as 3 µg of protein on surfaces and in hard to reach areas CleanTrace™ ALLTEC60 Surface Protein Swabs are a fast and easy way to visually check whether cleaning has been carried out to a satisfactory standard by means of protein detection. By comparing the colour of the swab and solution against the label, the user can easily interpret the results for the presence of allergens. Clean-Trace® ALTEC60 Surface Protein (Allergen) Test Swab is validated for a range of allergenic proteins, including egg, milk, gluten, soy, peanut, almond and buckwheat. Refrigeration is not required -swabs can be stored at room temperature (25°C or 77°F). This easy-to-use device requires minimal training. The results are semi-quantitative with four possible color interpretations. The faster the test turns purple and the stronger the color change the higher the level of contamination on a surface. Quick detection and confirmation allows immediate corrective action to take place. A visual reading of a colour change indicates the level of cleanliness by detecting as little as 3µg of allergen on surfaces and in solution. No instrumentation is required to interpret the sample results. This product is easily integrated into company HACCP programs to monitor for cleaning effectiveness. Its ease of use, reliable detection and fast, visible results can help in teaching staff the importance of cleaning. -
The complete 3M Sample Handling Collection. Better testing begins with better samples – and better samples are obtained when you use the best collection tools. 3M™ Sample Collection, Preparation and Processing solutions provide quality products for every step of your process. Convenient, ready-to-use products. Consistent, reliable results. Every time. Choosing a sampling sponge. When determining which sampling sponge to use, it’s important to consider certain factors in your testing process:- Bacteria
- Biocides
- Environment
- Strength
- Consistency
- Temperature
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