- Complement DNA microarrays. DNA microarrays allow scientists to measure the expression of thousands of genes of a cell under one growth condition, whereas Phenotype MicroArrays allow scientists to measure the effect of one gene in the cell under thousands of growth conditions.
- Provide rapid answers to critical questions about cellular properties in a simple, rapid, efficient, and cost- effective process.
- Provide rapid answers to how a gene of unknown function affects a cell at a physiological level.
- Provide cell-to-cell comparisons to find novel drug targets based on in vivo physiological differences.
- Provide a means to assay the effect of a drug on thousands of cellular targets under thousands of physiological states of the cell.
- Provide rapid answers to questions about how a new lead affects a cell at a biochemical level.
- Provide information on how new drug leads compare to and interact with existing drugs.
- Reduce the time and cost of slow and cumbersome animal, plant, or human studies in late development.
- Eliminate unproductive leads and projects, saving substantial R&D budget dollars that would be wasted in later development.
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Drug Discovery with Biolog Technologies BiOLOG’s Phenotype MicroArray™ Technology for Microbial and Mammalian Cells, and new MitoPlate™ assays are used by pharmaceutical companies, biotech’s and academic institutions to assist with new drug target identification and drug re-purposing, and any applications in the process of developing solutions for the treatment and curing of disease. Benefits of Phenotype MicroArrays in Drug Discovery: -
Phenotype MicroArrays for Mammalian Cells Using Biolog’s Phenotype MicroArray™ Technology, any type of cell can be characterised for metabolically related phenotypes in over 1,000 assays in a simple, rapid, and cost-effective manner. By measuring a cell’s metabolic activity, including responses to a genetic alteration or chemical stimuli, this integrated system of cellular assays, instrumentation, and bioinformatic software reveals unique and insightful information on metabolic pathway activities, and cellular sensitivity to nutrients, hormones, cytokines, anti-cancer agents, and ions. By measuring energy output of cells, the technology also provides new assays for understanding mitochondrial function and toxicity. Phenotype MicroArrays enable insight and discovery to expedite scientific publications. This is accomplished using 96 well microplates, pre-loaded with hundreds of KEGG pathway-matched metabolic substrates, and metabolic effectors. Phenotype MicroArrays are a proven method of cellular screening that is beneficial in a wide range of research applications:- Genotype/phenotype studies
- Cell line characterisation and QC
- Cellular metabolism, metabolic disorders, nutrition
- Cell energetics growth and death
- Hormone effects on cells
- Metabolic reprogramming in cancer, Warburg effect, anti-cancer drug sensitivity
- Mitochondrial toxicology in chemical/drug screens
- Stem cells and differentiation
- Cell line and bio process development
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Analysing Mitochondria at Higher Resolution with MitoPlate™ Technology Analysing Mitochondria at higher resolution with Biolog Technology MitoPlates™ provide a powerful new research tool by allowing scientists to run preconfigured sets of 96 mitochondrial function assays in one experiment. Mitochondria can be interrogated and characterised in novel ways, looking at rates of substrate metabolism, sensitivity to drugs and other chemicals, and effects of mutations in mitochondria-related genes. Assay Principle Mitochondrial function is assayed by measuring the rates of electron flow into and through the electron transport chain from metabolic substrates that produce NAD(P)H or FADH2 such as L-malate, succinate, pyruvate, etc. Each substrate follows a different route, using different transporters to enter the mitochondria and different dehydrogenases to produce NAD(P)H or FADH2, The electrons travel from the beginning (complex 1 or 2) to the distal portion of the electron transport chain where a tetrazolium redox dye (MC) acts as a terminal electron acceptor that turns purple upon reduction. Additional MitoPlate assays probe the sensitivity of the mitochondria to a set of 22 diverse inhibitors. Investigate how mitochondria change with:- Cell differentiation
- Cancer & Aging
- Neurological disorders
- Metabolic disorders
- Immune cell activation
- Bacterial/viral infection
- Inborn genetic defects
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Phenotype MicroArrays for Microbial Cells Biolog's Phenotype MicroArray™ Technology enables researchers to characterise cells in up to 1,920 assays and evaluate cell changes under thousands of culture conditions and physiological states in a simple, rapid, efficient and cost-effective manner. By measuring a cell’s response to a genetic or environmental alteration, this integrated system of cellular assays, instrumentation, and bioinformatics software reveals invaluable information to speed insight and discovery and expedite scientific publication. Phenotype MicroArrays (PMs) have broad applicability for genotype-phenotype characterisation as well as for determining optimal conditions for cellular growth, sporulation and germination, production of secondary metabolites, or enzymatic activities in cell lines. Phenotype MicroArrays are a proven method of cellular screening that is extremely beneficial in a wide range of research applications:
- Discovering effects of loss or gain of gene function.
- Measuring/documenting changes in cell metabolism over time or under different environmental conditions.
- Improvement and QC of phenotypic stability of cell lines.
- Improved efficacy in the production of compounds in biological fermentation processes.
- Evaluation of new drug/antibiotic candidates in toxicological profiling and mode of action studies.
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EcoPlates™ Examine environmental community diversity with Biolog EcoPlates™. Biolog EcoPlates provide a sensitive and reliable index of environmental change. This approach, called community-level physiological profiling (CLPP), is effective at distinguishing spatial and temporal changes in the metabolism of microbial communities. Microbial communities provide useful information about environmental change. Microorganisms are present in virtually all environments and are typically the first organisms to react to chemical and physical changes in the environment. Changes in microbial communities are often a precursor to changes in the health and viability of the environment as a whole. -
Microbial ID microplates from Biolog Biolog Solutions for Microbial Identification Rapidly and accurately identify nearly 3,000 species of aerobic and anaerobic bacteria, yeast, and fungi. In addition to species-level identification, Biolog’s advanced phenotypic technology provides valuable information on the functional properties of strains. Biolog’s carbon source utilisation technology identifies environmental and pathogenic microorganisms by producing a characteristic pattern or “metabolic fingerprint” from discrete test reactions performed within a 96-well microplate. Culture suspensions are tested with a panel of pre-selected assays, then incubated, read and compared to our extensive database. Biolog’s Microbial ID microplates incorporate a patented redox tetrazolium dye that changes colour as a result of cellular respiration, providing a metabolic fingerprint. Our complete solution includes instruments, consumables, databases, software. -
PreBioM™ Microplates Introducing The PreBioM™ line of microplates from Biolog. The microplates allow researchers studying human or animal gut microbiome communities to better understand metabolism of prebiotics. PreBioM plates offer a multidimensional phenotype profiling solution based on the impact of prebiotics on microbial function. The three-plate system contains 90 unique prebiotic substrates tailored to investigate the intricate interplay between prebiotics and the microbiome. These plates consist of a curated selection of prebiotic substrates of various molecular weights designed to characterise bacterial species and their metabolic pathways. The PreBioM plates, combined with the Biolog Odin family of instruments and software, provide a high throughput phenotypic solution compatible with a wide range of species, including the obligate anaerobes inhabiting the gut microbiome as well as microbial communities.